› 1hi1

X-ray diffraction
3Å resolution

RNA dependent RNA polymerase from dsRNA bacteriophage phi6 plus bound NTP

Released:
DOI: 10.2210/pdb1hi1/pdb
PDB entry 1hi1 coloured by chain, front view.
PDB entry 1hi1 coloured by chain, side view.
PDB entry 1hi1 coloured by chain, top view.
Source organism: Pseudomonas virus phi6
Primary publication:
A mechanism for initiating RNA-dependent RNA polymerization.
Butcher SJ, Grimes JM, Makeyev EV, Bamford DH, Stuart DI
Nature 410 235-40 (2001)
PMID: 11242087

Function and Biology Details

Reaction catalysed: 
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145660 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-directed RNA polymerase Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 664 amino acids
Theoretical weight: 74.9 KDa
Source organism: Pseudomonas virus phi6
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11124 (Residues: 2-665; Coverage: 100%)
Gene name: P2
Sequence domains: Viral RNA-dependent RNA polymerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand ATP 3 x ATP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 105.65Å b: 92.7Å c: 140.79Å
α: 90° β: 101.09° γ: 90°
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

68 review citations

Replication of hepatitis C virus.
Moradpour et al. (2007)
67 more

6 mentions without citation

Cystoviral RNA-directed RNA polymerases: Regulation of RNA synthesis on multiple time and length scales.
Alphonse et al. (2017)
5 more