› 1hj9

X-ray diffraction
0.95Å resolution

Atomic resolution structures of trypsin provide insight into structural radiation damage

Released:
DOI: 10.2210/pdb1hj9/pdb
PDB entry 1hj9 coloured by chain, front view.
PDB entry 1hj9 coloured by chain, side view.
PDB entry 1hj9 coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Atomic resolution structures of trypsin provide insight into structural radiation damage.
Leiros HK, McSweeney SM, Smalås AO
Acta Crystallogr D Biol Crystallogr 57 488-97 (2001)
PMID: 11264577

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Arg-|-, Lys-|-.
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-133319 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine protease 1 Chain: A
Molecule details ›
Chain: A
Length: 223 amino acids
Theoretical weight: 23.32 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P00760 (Residues: 24-246; Coverage: 97%)
Gene names: PRSS1, TRP1, TRY1, TRYP1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

4 bound ligands:
Ligand ANL 1 x ANL
Ligand SO4 3 x SO4
Ligand GOL 1 x GOL
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM1A
Spacegroup: P212121
Unit cell:
a: 54.055Å b: 56.812Å c: 66.282Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.117 not available 0.14

Quick links

Citations

26 citation in other articles

The catalytic pathway of horseradish peroxidase at high resolution.
Berglund et al. (2002)
25 more

8 mentions without citation

Phaser.MRage: automated molecular replacement.
Bunkóczi et al. (2013)
7 more