› 1hjq

X-ray diffraction
2.55Å resolution

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.

Released:
DOI: 10.2210/pdb1hjq/pdb
PDB entry 1hjq coloured by chain, front view.
PDB entry 1hjq coloured by chain, side view.
PDB entry 1hjq coloured by chain, top view.
Source organism: Humicola insolens
Primary publication:
Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.
Le Nours J, Ryttersgaard C, Lo Leggio L, Østergaard PR, Borchert TV, Christensen LL, Larsen S
Protein Sci 12 1195-204 (2003)
PMID: 12761390

Function and Biology Details

Reaction catalysed: 
The enzyme specifically hydrolyzes (1->4)-beta-D-galactosidic linkages in type I arabinogalactans.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-160720 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arabinogalactan endo-beta-1,4-galactanase Chain: A
Molecule details ›
Chain: A
Length: 332 amino acids
Theoretical weight: 37.69 KDa
Source organism: Humicola insolens
Expression system: Aspergillus oryzae
UniProt:
  • Canonical: P83691 (Residues: 1-332; Coverage: 100%)
Sequence domains: Glycosyl hydrolase family 53
Structure domains: Glycosidases

Ligands and Environments

1 bound ligand:
Ligand NAG 1 x NAG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P212121
Unit cell:
a: 45.254Å b: 68.955Å c: 135.16Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.247
Expression system: Aspergillus oryzae

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Citations

2 review citations

Myceliophthora thermophila syn. Sporotrichum thermophile: a thermophilic mould of biotechnological potential.
Singh B. (2016)
1 more

4 mentions without citation

Structure of two fungal beta-1,4-galactanases: searching for the basis for temperature and pH optimum.
Le Nours et al. (2003)
3 more