› 1hk0

X-ray diffraction
1.25Å resolution

Human GammaD Crystallin Structure at 1.25 A Resolution

Released:
DOI: 10.2210/pdb1hk0/pdb
PDB entry 1hk0 coloured by chain, front view.
PDB entry 1hk0 coloured by chain, side view.
PDB entry 1hk0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
High-resolution X-ray crystal structures of human gammaD crystallin (1.25 A) and the R58H mutant (1.15 A) associated with aculeiform cataract.
Basak A, Bateman O, Slingsby C, Pande A, Asherie N, Ogun O, Benedek GB, Pande J
J Mol Biol 328 1137-47 (2003)
PMID: 12729747

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139502 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Gamma-crystallin D Chain: X
Molecule details ›
Chain: X
Length: 173 amino acids
Theoretical weight: 20.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P07320 (Residues: 2-174; Coverage: 99%)
Gene names: CRYG4, CRYGD
Sequence domains: Beta/Gamma crystallin
Structure domains: Crystallins

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-1
Spacegroup: P212121
Unit cell:
a: 33.22Å b: 52.971Å c: 90.468Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.164 0.207
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

7 review citations

Ageing and vision: structure, stability and function of lens crystallins.
Bloemendal et al. (2004)
6 more

76 mentions without citation

Protein misfolding and aggregation in cataract disease and prospects for prevention.
Moreau et al. (2012)
75 more