› 1hkb

X-ray diffraction
2.8Å resolution

CRYSTAL STRUCTURE OF RECOMBINANT HUMAN BRAIN HEXOKINASE TYPE I COMPLEXED WITH GLUCOSE AND GLUCOSE-6-PHOSPHATE

Released:
DOI: 10.2210/pdb1hkb/pdb
PDB entry 1hkb coloured by chain, front view.
PDB entry 1hkb coloured by chain, side view.
PDB entry 1hkb coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
The mechanism of regulation of hexokinase: new insights from the crystal structure of recombinant human brain hexokinase complexed with glucose and glucose-6-phosphate.
Aleshin AE, Zeng C, Bourenkov GP, Bartunik HD, Fromm HJ, Honzatko RB
Structure 6 39-50 (1998)
PMID: 9493266

Function and Biology Details

Reaction catalysed: 
ATP + D-hexose = ADP + D-hexose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148563 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hexokinase-1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 917 amino acids
Theoretical weight: 102.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19367 (Residues: 1-917; Coverage: 100%)
Gene name: HK1
Sequence domains:
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand BGC 4 x BGC
Ligand G6P 4 x G6P
Ligand CA 4 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 83Å b: 122Å c: 123Å
α: 90° β: 92° γ: 90°
R-values:
R R work R free
0.2 0.2 0.27
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Glucose Transporters at the Blood-Brain Barrier: Function, Regulation and Gateways for Drug Delivery.
Patching SG. (2017)
7 more

6 mentions without citation

A dominant mutation in hexokinase 1 (HK1) causes retinitis pigmentosa.
Sullivan et al. (2014)
5 more