› 1hlu

X-ray diffraction
2.65Å resolution

STRUCTURE OF BOVINE BETA-ACTIN-PROFILIN COMPLEX WITH ACTIN BOUND ATP PHOSPHATES SOLVENT ACCESSIBLE

Released:
DOI: 10.2210/pdb1hlu/pdb
PDB entry 1hlu coloured by chain, front view.
PDB entry 1hlu coloured by chain, side view.
PDB entry 1hlu coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
The structure of an open state of beta-actin at 2.65 A resolution.
Chik JK, Lindberg U, Schutt CE
J Mol Biol 263 607-23 (1996)
PMID: 8918942

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-135936 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Actin, cytoplasmic 1 Chain: A
Molecule details ›
Chain: A
Length: 375 amino acids
Theoretical weight: 41.69 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P60712 (Residues: 2-375; Coverage: 100%)
Gene name: ACTB
Sequence domains: Actin
Structure domains:
Profilin-1 Chain: P
Molecule details ›
Chain: P
Length: 140 amino acids
Theoretical weight: 14.97 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02584 (Residues: 2-140; Coverage: 99%)
Gene name: PFN1
Sequence domains: Profilin
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

2 bound ligands:
Ligand CA 1 x CA
Ligand ATP 1 x ATP
1 modified residue:
Ligand HIC 1 x HIC

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 38.14Å b: 72.24Å c: 185.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.33

Quick links

Citations

17 review citations

Structural mechanism of muscle contraction.
Geeves et al. (1999)
16 more

31 mentions without citation

The state of the filament.
Aguda et al. (2005)
30 more