› 1hqc

X-ray diffraction
3.2Å resolution

STRUCTURE OF RUVB FROM THERMUS THERMOPHILUS HB8

Released:
DOI: 10.2210/pdb1hqc/pdb
PDB entry 1hqc coloured by chain, front view.
PDB entry 1hqc coloured by chain, side view.
PDB entry 1hqc coloured by chain, top view.
Source organism: Thermus thermophilus
Primary publication:
Crystal structure of the Holliday junction migration motor protein RuvB from Thermus thermophilus HB8.
Yamada K, Kunishima N, Mayanagi K, Ohnishi T, Nishino T, Iwasaki H, Shinagawa H, Morikawa K
Proc Natl Acad Sci U S A 98 1442-7 (2001)
PMID: 11171970

Function and Biology Details

Reaction catalysed: 
ATP + H(2)O = ADP + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178141 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Holliday junction branch migration complex subunit RuvB Chains: A, B
Molecule details ›
Chains: A, B
Length: 324 amino acids
Theoretical weight: 36.05 KDa
Source organism: Thermus thermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5SL87 (Residues: 1-324; Coverage: 100%)
Gene names: TTHA0406, ruvB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand ADE 2 x ADE
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL24XU
Spacegroup: P43212
Unit cell:
a: 84.865Å b: 84.865Å c: 355.24Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.263 0.263 0.296
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Structure and mechanism of helicases and nucleic acid translocases.
Singleton et al. (2007)
16 more

10 mentions without citation

The AAA+ superfamily of functionally diverse proteins.
Snider et al. (2008)
9 more