› 1hqo

X-ray diffraction
2.3Å resolution

CRYSTAL STRUCTURE OF THE NITROGEN REGULATION FRAGMENT OF THE YEAST PRION PROTEIN URE2P

Released:
DOI: 10.2210/pdb1hqo/pdb
PDB entry 1hqo coloured by chain, front view.
PDB entry 1hqo coloured by chain, side view.
PDB entry 1hqo coloured by chain, top view.
Source organism: Saccharomyces cerevisiae
Primary publication:
The crystal structure of the nitrogen regulation fragment of the yeast prion protein Ure2p.
Umland TC, Taylor KL, Rhee S, Wickner RB, Davies DR
Proc Natl Acad Sci U S A 98 1459-64 (2001)
PMID: 11171973

Function and Biology Details

Reaction catalysed: 
2 glutathione + H(2)O(2) = glutathione disulfide + 2 H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149892 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transcriptional regulator URE2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.94 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P23202 (Residues: 97-354; Coverage: 73%)
Gene names: N1165, URE2, YNL229C
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 14 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P212121
Unit cell:
a: 64.31Å b: 69.19Å c: 149.99Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.221 0.275
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Prions in yeast.
Liebman et al. (2012)
9 more

4 mentions without citation

The crystal structure of the nitrogen regulation fragment of the yeast prion protein Ure2p.
Umland et al. (2001)
3 more