› 1hye

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE MJ0490 GENE PRODUCT, THE FAMILY OF LACTATE/MALATE DEHYDROGENASE, DIMERIC STRUCTURE

Released:
DOI: 10.2210/pdb1hye/pdb
PDB entry 1hye coloured by chain, front view.
PDB entry 1hye coloured by chain, side view.
PDB entry 1hye coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Crystal structure of the MJ0490 gene product of the hyperthermophilic archaebacterium Methanococcus jannaschii, a novel member of the lactate/malate family of dehydrogenases.
Lee BI, Chang C, Cho SJ, Eom SH, Kim KK, Yu YG, Suh SW
J Mol Biol 307 1351-62 (2001)
PMID: 11292347

Function and Biology Details

Reaction catalysed: 
A (2S)-2-hydroxycarboxylate + NAD(P)(+) = a 2-oxocarboxylate + NAD(P)H
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-178759 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-2-hydroxycarboxylate dehydrogenase (NAD(P)(+)) Chain: A
Molecule details ›
Chain: A
Length: 313 amino acids
Theoretical weight: 34.66 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: Q60176 (Residues: 1-313; Coverage: 100%)
Gene names: MJ0490, mdh, mdhB
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAP 1 x NAP
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P21212
Unit cell:
a: 47.647Å b: 125.101Å c: 58.082Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.228
Expression system: Escherichia coli

Quick links

Citations

15 citation in other articles

A structurally conserved water molecule in Rossmann dinucleotide-binding domains.
Bottoms et al. (2002)
14 more

1 mention without citation

4SCOPmap: automated assignment of protein structures to evolutionary superfamilies.
Cheek et al. (2004)