› 1hyh

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF L-2-HYDROXYISOCAPROATE DEHYDROGENASE FROM LACTOBACILLUS CONFUSUS AT 2.2 ANGSTROMS RESOLUTION-AN EXAMPLE OF STRONG ASYMMETRY BETWEEN SUBUNITS

Released:
DOI: 10.2210/pdb1hyh/pdb
PDB entry 1hyh coloured by chain, front view.
PDB entry 1hyh coloured by chain, side view.
PDB entry 1hyh coloured by chain, top view.
Source organism: Weissella confusa
Primary publication:
Crystal structure of L-2-hydroxyisocaproate dehydrogenase from Lactobacillus confusus at 2.2 A resolution. An example of strong asymmetry between subunits.
Niefind K, Hecht HJ, Schomburg D
J Mol Biol 251 256-81 (1995)
PMID: 7643402

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146898 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-2-hydroxyisocaproate dehydrogenase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 309 amino acids
Theoretical weight: 33.08 KDa
Source organism: Weissella confusa
Expression system: Escherichia coli
UniProt:
  • Canonical: P14295 (Residues: 2-310; Coverage: 100%)
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 4 x SO4
Ligand NAD 4 x NAD
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P3221
Unit cell:
a: 135.9Å b: 135.9Å c: 205.9Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 not available 0.25
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Comparative genomics of enzymes in flavor-forming pathways from amino acids in lactic acid bacteria.
Liu et al. (2008)
2 more

2 mentions without citation

An Ancient Fingerprint Indicates the Common Ancestry of Rossmann-Fold Enzymes Utilizing Different Ribose-Based Cofactors.
Laurino et al. (2016)
1 more