› 1i10

X-ray diffraction
2.3Å resolution

HUMAN MUSCLE L-LACTATE DEHYDROGENASE M CHAIN, TERNARY COMPLEX WITH NADH AND OXAMATE

Released:
DOI: 10.2210/pdb1i10/pdb
PDB entry 1i10 coloured by chain, front view.
PDB entry 1i10 coloured by chain, side view.
PDB entry 1i10 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for altered activity of M- and H-isozyme forms of human lactate dehydrogenase.
Read JA, Winter VJ, Eszes CM, Sessions RB, Brady RL
Proteins 43 175-85 (2001)
PMID: 11276087

Function and Biology Details

Reaction catalysed: 
(S)-lactate + NAD(+) = pyruvate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132412 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-lactate dehydrogenase A chain Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 331 amino acids
Theoretical weight: 36.6 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P00338 (Residues: 2-332; Coverage: 100%)
Gene names: LDHA, PIG19
Sequence domains:
Structure domains:

Ligands and Environments


Cofactor: Ligand NAI 8 x NAI
2 bound ligands:
Ligand ACT 8 x ACT
Ligand OXM 8 x OXM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SRS BEAMLINE PX7.2
Spacegroup: P212121
Unit cell:
a: 64.942Å b: 158.542Å c: 266.226Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.2 0.197 0.257
Expression system: Escherichia coli BL21

Quick links

Citations

21 review citations

The Regulation and Function of Lactate Dehydrogenase A: Therapeutic Potential in Brain Tumor.
Valvona et al. (2016)
20 more

34 mentions without citation

Small-molecule inhibitors of human LDH5.
Granchi et al. (2013)
33 more