› 1i1x

X-ray diffraction
1.11Å resolution

1.11 A ATOMIC RESOLUTION STRUCTURE OF A THERMOSTABLE XYLANASE FROM THERMOASCUS AURANTIACUS

Released:
DOI: 10.2210/pdb1i1x/pdb
PDB entry 1i1x coloured by chain, front view.
PDB entry 1i1x coloured by chain, side view.
PDB entry 1i1x coloured by chain, top view.
Source organism: Thermoascus aurantiacus
Primary publication:
Thermostable xylanase from Thermoascus aurantiacus at ultrahigh resolution (0.89 A) at 100 K and atomic resolution (1.11 A) at 293 K refined anisotropically to small-molecule accuracy.
Natesh R, Manikandan K, Bhanumoorthy P, Viswamitra MA, Ramakumar S
Acta Crystallogr D Biol Crystallogr 59 105-17 (2003)
PMID: 12499546

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-xylosidic linkages in xylans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-149932 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Endo-1,4-beta-xylanase Chain: A
Molecule details ›
Chain: A
Length: 303 amino acids
Theoretical weight: 32.87 KDa
Source organism: Thermoascus aurantiacus
UniProt:
  • Canonical: P23360 (Residues: 27-329; Coverage: 100%)
Gene name: XYNA
Sequence domains: Glycosyl hydrolase family 10
Structure domains: Glycosidases

Ligands and Environments

No bound ligands
1 modified residue:
Ligand PCA 1 x PCA

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P21
Unit cell:
a: 41.52Å b: 68.09Å c: 51.44Å
α: 90° β: 113.56° γ: 90°
R-values:
R R work R free
0.099 0.099 0.124

Quick links

Citations

2 review citations

Microbial hemicellulases.
Shallom et al. (2003)
1 more