› 1i3j

X-ray diffraction
2.2Å resolution

CRYSTAL STRUCTURE OF THE DNA-BINDING DOMAIN OF INTRON ENDONUCLEASE I-TEVI WITH ITS SUBSTRATE

Released:
DOI: 10.2210/pdb1i3j/pdb
PDB entry 1i3j coloured by chain, front view.
PDB entry 1i3j coloured by chain, side view.
PDB entry 1i3j coloured by chain, top view.
Source organism: Tequatrovirus T4
Primary publication:
Intertwined structure of the DNA-binding domain of intron endonuclease I-TevI with its substrate.
Van Roey P, Waddling CA, Fox KM, Belfort M, Derbyshire V
EMBO J 20 3631-7 (2001)
PMID: 11447104

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113938 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Intron-associated endonuclease 1 Chain: A
Molecule details ›
Chain: A
Length: 116 amino acids
Theoretical weight: 13.24 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P13299 (Residues: 130-245; Coverage: 47%)
Gene name: ITEVIR
Sequence domains: Intron-encoded endonuclease 1, DNA-binding domain
5'-D(*TP*TP*CP*TP*TP*GP*GP*GP*TP*CP*TP*AP*CP*CP*GP*TP*TP*TP*AP*AP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 21 nucleotides
Theoretical weight: 6.41 KDa
Source organism: Tequatrovirus T4
Expression system: Not provided
5'-D(*AP*AP*TP*TP*AP*AP*AP*CP*GP*GP*TP*AP*GP*AP*CP*CP*CP*AP*AP*GP*A)-3' Chain: C
Molecule details ›
Chain: C
Length: 21 nucleotides
Theoretical weight: 6.47 KDa
Source organism: Tequatrovirus T4
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P21
Unit cell:
a: 55.14Å b: 65.21Å c: 43.67Å
α: 90° β: 93.1° γ: 90°
R-values:
R R work R free
0.215 0.215 0.248
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

11 review citations

Origins of specificity in protein-DNA recognition.
Rohs et al. (2010)
10 more

11 mentions without citation

Bacteriophage T4 genome.
Miller et al. (2003)
10 more