› 1i4s

X-ray diffraction
2.15Å resolution

CRYSTAL STRUCTURE OF RNASE III ENDONUCLEASE DOMAIN FROM AQUIFEX AEOLICUS AT 2.15 ANGSTROM RESOLUTION

Released:
DOI: 10.2210/pdb1i4s/pdb
PDB entry 1i4s coloured by chain, front view.
PDB entry 1i4s coloured by chain, side view.
PDB entry 1i4s coloured by chain, top view.
Source organism: Aquifex aeolicus
Primary publication:
Crystallographic and modeling studies of RNase III suggest a mechanism for double-stranded RNA cleavage.
Blaszczyk J, Tropea JE, Bubunenko M, Routzahn KM, Waugh DS, Court DL, Ji X
Structure 9 1225-36 (2001)
PMID: 11738048

Function and Biology Details

Reaction catalysed: 
Endonucleolytic cleavage to 5'-phosphomonoester.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-130429 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribonuclease 3 Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 17.28 KDa
Source organism: Aquifex aeolicus
Expression system: Escherichia coli
UniProt:
  • Canonical: O67082 (Residues: 1-147; Coverage: 67%)
Gene names: aq_946, rnc
Sequence domains: Ribonuclease III domain
Structure domains: Ribonuclease III domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X9B
Spacegroup: P212121
Unit cell:
a: 46.157Å b: 51.188Å c: 123.468Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.212 0.275
Expression system: Escherichia coli

Quick links

Citations

45 review citations

MicroRNAs: small RNAs with a big role in gene regulation.
He et al. (2004)
44 more

3 mentions without citation

Nanoscale dewetting transition in protein complex folding.
Hua et al. (2007)
2 more