› 1i5l

X-ray diffraction
2.75Å resolution

CRYSTAL STRUCTURE OF AN SM-LIKE PROTEIN (AF-SM1) FROM ARCHAEOGLOBUS FULGIDUS COMPLEXED WITH SHORT POLY-U RNA

Released:
DOI: 10.2210/pdb1i5l/pdb
PDB entry 1i5l coloured by chain, front view.
PDB entry 1i5l coloured by chain, side view.
PDB entry 1i5l coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
RNA binding in an Sm core domain: X-ray structure and functional analysis of an archaeal Sm protein complex.
Törö I, Thore S, Mayer C, Basquin J, Séraphin B, Suck D
EMBO J 20 2293-303 (2001)
PMID: 11331594

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128122 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Putative snRNP Sm-like protein Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Molecule details ›
Chains: A, B, C, D, E, F, G, H, I, J, K, L, M, N
Length: 77 amino acids
Theoretical weight: 8.39 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29386 (Residues: 1-77; Coverage: 100%)
Gene name: AF_0875
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
5'-R(*UP*UP*U)-3' Chains: U, Y
Molecule details ›
Chains: U, Y
Length: 3 nucleotides
Theoretical weight: 874 Da
Source organism: Archaeoglobus fulgidus
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand URI 4 x URI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID13
Spacegroup: P21
Unit cell:
a: 69.858Å b: 130.438Å c: 70.047Å
α: 90° β: 115.36° γ: 90°
R-values:
R R work R free
0.229 0.225 0.3
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

13 review citations

The bacterial Sm-like protein Hfq: a key player in RNA transactions.
Valentin-Hansen et al. (2004)
12 more

3 mentions without citation

Evolutionary diversification of the Sm family of RNA-associated proteins.
Scofield et al. (2008)
2 more