› 1i6u

X-ray diffraction
2.6Å resolution

RNA-PROTEIN INTERACTIONS: THE CRYSTAL STRUCTURE OF RIBOSOMAL PROTEIN S8/RRNA COMPLEX FROM METHANOCOCCUS JANNASCHII

Released:
DOI: 10.2210/pdb1i6u/pdb
PDB entry 1i6u coloured by chain, front view.
PDB entry 1i6u coloured by chain, side view.
PDB entry 1i6u coloured by chain, top view.
Source organism: Methanocaldococcus jannaschii
Primary publication:
Detailed analysis of RNA-protein interactions within the ribosomal protein S8-rRNA complex from the archaeon Methanococcus jannaschii.
Tishchenko S, Nikulin A, Fomenkova N, Nevskaya N, Nikonov O, Dumas P, Moine H, Ehresmann B, Ehresmann C, Piendl W, Lamzin V, Garber M, Nikonov S
J Mol Biol 311 311-24 (2001)
PMID: 11478863

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-156929 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Small ribosomal subunit protein uS8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 130 amino acids
Theoretical weight: 14.74 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Escherichia coli
UniProt:
  • Canonical: P54041 (Residues: 1-130; Coverage: 100%)
Gene names: MJ0470, rps8
Sequence domains: Ribosomal protein S8
Structure domains:
16S RRNA FRAGMENT Chains: C, D
Molecule details ›
Chains: C, D
Length: 37 nucleotides
Theoretical weight: 11.89 KDa
Source organism: Methanocaldococcus jannaschii
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE BM30A, EMBL/DESY, HAMBURG BEAMLINE X11
Spacegroup: P43212
Unit cell:
a: 121.687Å b: 121.687Å c: 137.975Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.218 0.251
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

2 review citations

Regulation of translation initiation by RNA binding proteins.
Babitzke et al. (2009)
1 more

18 mentions without citation

Computational approaches to 3D modeling of RNA.
Laing et al. (2010)
17 more