› 1i7d

X-ray diffraction
2.05Å resolution

NONCOVALENT COMPLEX OF E.COLI DNA TOPOISOMERASE III WITH AN 8-BASE SINGLE-STRANDED DNA OLIGONUCLEOTIDE

Released:
DOI: 10.2210/pdb1i7d/pdb
PDB entry 1i7d coloured by chain, front view.
PDB entry 1i7d coloured by chain, side view.
PDB entry 1i7d coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Crystal structure of a complex of a type IA DNA topoisomerase with a single-stranded DNA molecule.
Changela A, DiGate RJ, Mondragón A
Nature 411 1077-81 (2001)
PMID: 11429611

Function and Biology Details

Reaction catalysed: 
ATP-independent breakage of single-stranded DNA, followed by passage and rejoining
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113946 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
DNA topoisomerase 3 Chain: A
Molecule details ›
Chain: A
Length: 659 amino acids
Theoretical weight: 74.14 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P14294 (Residues: 1-653; Coverage: 100%)
Gene names: JW1752, b1763, topB
Sequence domains:
Structure domains:
5'-D(*CP*GP*CP*AP*AP*CP*TP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 8 nucleotides
Theoretical weight: 2.39 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand CL 1 x CL
Ligand SO4 1 x SO4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B
Spacegroup: C2
Unit cell:
a: 122.011Å b: 60.831Å c: 125.358Å
α: 90° β: 90.73° γ: 90°
R-values:
R R work R free
0.233 0.233 0.26
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

14 review citations

All tangled up: how cells direct, manage and exploit topoisomerase function.
Vos et al. (2011)
13 more

12 mentions without citation

Structural studies of type I topoisomerases.
Baker et al. (2009)
11 more