› 1i8t

X-ray diffraction
2.4Å resolution

STRUCTURE OF UDP-GALACTOPYRANOSE MUTASE FROM E.COLI

Released:
DOI: 10.2210/pdb1i8t/pdb
PDB entry 1i8t coloured by chain, front view.
PDB entry 1i8t coloured by chain, side view.
PDB entry 1i8t coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
UDP-galactopyranose mutase has a novel structure and mechanism.
Sanders DA, Staines AG, McMahon SA, McNeil MR, Whitfield C, Naismith JH
Nat Struct Biol 8 858-63 (2001)
PMID: 11573090

Function and Biology Details

Reaction catalysed: 
UDP-alpha-D-galactopyranose = UDP-alpha-D-galactofuranose
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
UDP-galactopyranose mutase Chains: A, B
Molecule details ›
Chains: A, B
Length: 367 amino acids
Theoretical weight: 43.03 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P37747 (Residues: 1-367; Coverage: 100%)
Gene names: JW2021, b2036, glf, yefE
Sequence domains:
Structure domains: NAD(P)-binding Rossmann-like Domain

Ligands and Environments


Cofactor: Ligand FAD 2 x FAD
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-2, null
Spacegroup: P212121
Unit cell:
a: 56.642Å b: 98.123Å c: 132.476Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.185 0.185 0.245
Expression system: Escherichia coli

Quick links

Citations

10 review citations

Chemistry and biology of galactofuranose-containing polysaccharides.
Richards et al. (2009)
9 more

12 mentions without citation

Structure, mechanism, and dynamics of UDP-galactopyranose mutase.
Tanner et al. (2014)
11 more