› 1i92

X-ray diffraction
1.7Å resolution

STRUCTURAL BASIS OF THE NHERF PDZ1-CFTR INTERACTION

Released:
DOI: 10.2210/pdb1i92/pdb
PDB entry 1i92 coloured by chain, front view.
PDB entry 1i92 coloured by chain, side view.
PDB entry 1i92 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis of the Na+/H+ exchanger regulatory factor PDZ1 interaction with the carboxyl-terminal region of the cystic fibrosis transmembrane conductance regulator.
Karthikeyan S, Leung T, Ladias JA
J Biol Chem 276 19683-6 (2001)
PMID: 11304524

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127084 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Na(+)/H(+) exchange regulatory cofactor NHE-RF1 Chain: A
Molecule details ›
Chain: A
Length: 91 amino acids
Theoretical weight: 9.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O14745 (Residues: 11-99; Coverage: 25%)
Gene names: NHERF, NHERF1, SLC9A3R1
Sequence domains: PDZ domain
Structure domains: Pdz3 Domain

Ligands and Environments

1 bound ligand:
Ligand CL 2 x CL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU300
Spacegroup: P3121
Unit cell:
a: 51.658Å b: 51.658Å c: 66.966Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.21 0.187 0.217
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

13 review citations

PDZ domain proteins: plug and play!
Nourry et al. (2003)
12 more

13 mentions without citation

A specificity map for the PDZ domain family.
Tonikian et al. (2008)
12 more