› 1i9j

X-ray diffraction
2.6Å resolution

TESTOSTERONE COMPLEX STRUCTURE OF THE RECOMBINANT MONOCLONAL WILD TYPE ANTI-TESTOSTERONE FAB FRAGMENT

Released:
DOI: 10.2210/pdb1i9j/pdb
PDB entry 1i9j coloured by chain, front view.
PDB entry 1i9j coloured by chain, side view.
PDB entry 1i9j coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Structural insights into steroid hormone binding: the crystal structure of a recombinant anti-testosterone Fab fragment in free and testosterone-bound forms.
Valjakka J, Takkinenz K, Teerinen T, Söderlund H, Rouvinen J
J Biol Chem 277 4183-90 (2002)
PMID: 11707437

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-179239 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Ig-like domain-containing protein Chain: L
Molecule details ›
Chain: L
Length: 219 amino acids
Theoretical weight: 24.06 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q65ZC0 (Residues: 1-219; Coverage: 100%)
Sequence domains:
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: H
Molecule details ›
Chain: H
Length: 220 amino acids
Theoretical weight: 23.51 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q91Z05 (Residues: 20-120, 121-236; Coverage: 48%)
Gene names: AU044919, Ighg
Sequence domains:
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand TES 1 x TES
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200HB
Spacegroup: P21212
Unit cell:
a: 89.866Å b: 95.564Å c: 67.218Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.188 0.255
Expression system: Escherichia coli

Quick links

Citations

13 citation in other articles

Cross-reactivity of steroid hormone immunoassays: clinical significance and two-dimensional molecular similarity prediction.
Krasowski et al. (2014)
12 more

5 mentions without citation

Crystal structure of the HIV-2 neutralizing Fab fragment 7C8 with high specificity to the V3 region of gp125.
Uchtenhagen et al. (2011)
4 more