› 1i9r

X-ray diffraction
3.1Å resolution

STRUCTURE OF CD40L IN COMPLEX WITH THE FAB FRAGMENT OF HUMANIZED 5C8 ANTIBODY

Released:
DOI: 10.2210/pdb1i9r/pdb
PDB entry 1i9r coloured by chain, front view.
PDB entry 1i9r coloured by chain, side view.
PDB entry 1i9r coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of CD40 ligand in complex with the Fab fragment of a neutralizing humanized antibody.
Karpusas M, Lucci J, Ferrant J, Benjamin C, Taylor FR, Strauch K, Garber E, Hsu YM
Structure 9 321-9 (2001)
PMID: 11525169

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero nonamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-225573 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
CD40 ligand, soluble form Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 146 amino acids
Theoretical weight: 15.81 KDa
Source organism: Homo sapiens
Expression system: Komagataella pastoris
UniProt:
  • Canonical: P29965 (Residues: 116-261; Coverage: 56%)
Gene names: CD40L, CD40LG, TNFSF5, TRAP
Sequence domains: TNF(Tumour Necrosis Factor) family
Structure domains: Jelly Rolls
IMMUNOGLOBULIN H Chains: H, K, X
Molecule details ›
Chains: H, K, X
Length: 219 amino acids
Theoretical weight: 23.3 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins
IMMUNOGLOBULIN L Chains: L, M, Y
Molecule details ›
Chains: L, M, Y
Length: 218 amino acids
Theoretical weight: 23.88 KDa
Source organism: Homo sapiens
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand ZN 3 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2
Unit cell:
a: 224.48Å b: 129.91Å c: 96.49Å
α: 90° β: 109.6° γ: 90°
R-values:
R R work R free
0.233 0.233 0.285
Expression system: Komagataella pastoris

Quick links

Citations

5 review citations

Gene therapy progress and prospects: RNA aptamers.
Que-Gewirth et al. (2007)
4 more

27 mentions without citation

Benchmarking and analysis of protein docking performance in Rosetta v3.2.
Chaudhury et al. (2011)
26 more