› 1ia9

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE OF THE ATYPICAL PROTEIN KINASE DOMAIN OF A TRP CA-CHANNEL, CHAK (AMPPNP COMPLEX)

Released:
DOI: 10.2210/pdb1ia9/pdb
PDB entry 1ia9 coloured by chain, front view.
PDB entry 1ia9 coloured by chain, side view.
PDB entry 1ia9 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity.
Yamaguchi H, Matsushita M, Nairn AC, Kuriyan J
Mol Cell 7 1047-57 (2001)
PMID: 11389851

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transient receptor potential cation channel subfamily M member 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 32.25 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q923J1 (Residues: 1549-1828; Coverage: 15%)
Gene names: Chak, Ltrpc7, Trpm7
Sequence domains: Alpha-kinase family
Structure domains:

Ligands and Environments

3 bound ligands:
Ligand ZN 2 x ZN
Ligand ANP 2 x ANP
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 109.27Å b: 136.55Å c: 113.26Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.243 0.24 0.294
Expression system: Spodoptera frugiperda

Quick links

Citations

66 review citations

TRP channels as cellular sensors.
Clapham DE. (2003)
65 more

20 mentions without citation

Design principles underpinning the regulatory diversity of protein kinases.
Oruganty et al. (2012)
19 more