› 1iah

X-ray diffraction
2.4Å resolution

CRYSTAL STRUCTURE OF THE ATYPICAL PROTEIN KINASE DOMAIN OF A TRP CA-CHANNEL, CHAK (ADP-MG COMPLEX)

Released:
DOI: 10.2210/pdb1iah/pdb
PDB entry 1iah coloured by chain, front view.
PDB entry 1iah coloured by chain, side view.
PDB entry 1iah coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structure of the atypical protein kinase domain of a TRP channel with phosphotransferase activity.
Yamaguchi H, Matsushita M, Nairn AC, Kuriyan J
Mol Cell 7 1047-57 (2001)
PMID: 11389851

Function and Biology Details

Reaction catalysed: 
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187659 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Transient receptor potential cation channel subfamily M member 7 Chains: A, B
Molecule details ›
Chains: A, B
Length: 280 amino acids
Theoretical weight: 32.25 KDa
Source organism: Mus musculus
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q923J1 (Residues: 1549-1828; Coverage: 15%)
Gene names: Chak, Ltrpc7, Trpm7
Sequence domains: Alpha-kinase family
Structure domains:

Ligands and Environments

4 bound ligands:
Ligand ZN 2 x ZN
Ligand ADP 2 x ADP
Ligand MG 1 x MG
Ligand DTT 1 x DTT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2221
Unit cell:
a: 108.961Å b: 135.458Å c: 113.204Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.217 0.215 0.249
Expression system: Spodoptera frugiperda

Quick links

Citations

68 review citations

TRP channels as cellular sensors.
Clapham DE. (2003)
67 more

7 mentions without citation

eEF2K Inhibitor Design: The Progression of Exemplary Structure-Based Drug Design.
Klupt et al. (2023)
6 more