› 1icw

X-ray diffraction
2.01Å resolution

INTERLEUKIN-8, MUTANT WITH GLU 38 REPLACED BY CYS AND CYS 50 REPLACED BY ALA

Released:
DOI: 10.2210/pdb1icw/pdb
PDB entry 1icw coloured by chain, front view.
PDB entry 1icw coloured by chain, side view.
PDB entry 1icw coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural change and receptor binding in a chemokine mutant with a rearranged disulfide: X-ray structure of E38C/C50AIL-8 at 2 A resolution.
Eigenbrot C, Lowman HB, Chee L, Artis DR
Proteins 27 556-66 (1997)
PMID: 9141135

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145181 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Interleukin-8 Chains: A, B
Molecule details ›
Chains: A, B
Length: 72 amino acids
Theoretical weight: 8.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P10145 (Residues: 28-99; Coverage: 91%)
Gene names: CXCL8, IL8
Sequence domains: Small cytokines (intecrine/chemokine), interleukin-8 like
Structure domains: OB fold (Dihydrolipoamide Acetyltransferase, E2P)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Spacegroup: P212121
Unit cell:
a: 46.4Å b: 49.2Å c: 69.5Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.194 0.194 0.266
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Structure, function, and inhibition of chemokines.
Fernandez et al. (2002)
2 more

10 mentions without citation

Role of the CXCL8-CXCR1/2 Axis in Cancer and Inflammatory Diseases.
Ha et al. (2017)
9 more