› 1ig4

Solution NMR

Solution Structure of the Methyl-CpG-Binding Domain of Human MBD1 in Complex with Methylated DNA

Released:
DOI: 10.2210/pdb1ig4/pdb
PDB entry 1ig4 coloured by chain, ensemble of 20 models, front view.
PDB entry 1ig4 coloured by chain, ensemble of 20 models, side view.
PDB entry 1ig4 coloured by chain, ensemble of 20 models, top view.
Source organism: Homo sapiens
Primary publication:
Solution structure of the methyl-CpG binding domain of human MBD1 in complex with methylated DNA.
Ohki I, Shimotake N, Fujita N, Jee J, Ikegami T, Nakao M, Shirakawa M
Cell 105 487-97 (2001)
PMID: 11371345

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-113957 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Methyl-CpG-binding domain protein 1 Chain: A
Molecule details ›
Chain: A
Length: 75 amino acids
Theoretical weight: 8.52 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UIS9 (Residues: 1-75; Coverage: 12%)
Gene names: CXXC3, MBD1, PCM1
Sequence domains: Methyl-CpG binding domain
Structure domains: Methyl-cpg-binding Protein 2; Chain A
5'-D(*GP*TP*AP*TP*CP*(5CM)P*GP*GP*AP*TP*AP*C)-3' Chains: B, C
Molecule details ›
Chains: B, C
Length: 12 nucleotides
Theoretical weight: 3.68 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand 5CM 2 x 5CM

Experiments and Validation Details

Entry percentile scores
Refinement method: Structure calculations were performed following simulated annealing protocols using X-PLOR.
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

44 review citations

Heterochromatin revisited.
Grewal et al. (2007)
43 more

15 mentions without citation

A Structural Perspective on Readout of Epigenetic Histone and DNA Methylation Marks.
Patel DJ. (2016)
14 more