› 1ikn

X-ray diffraction
2.3Å resolution

IKAPPABALPHA/NF-KAPPAB COMPLEX

Released:
DOI: 10.2210/pdb1ikn/pdb
PDB entry 1ikn coloured by chain, front view.
PDB entry 1ikn coloured by chain, side view.
PDB entry 1ikn coloured by chain, top view.
Source organisms:
Primary publication:
The crystal structure of the IkappaBalpha/NF-kappaB complex reveals mechanisms of NF-kappaB inactivation.
Huxford T, Huang DB, Malek S, Ghosh G
Cell 95 759-70 (1998)
PMID: 9865694

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-150584 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Transcription factor p65 Chain: A
Molecule details ›
Chain: A
Length: 286 amino acids
Theoretical weight: 32.74 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: Q04207 (Residues: 19-304; Coverage: 52%)
Gene names: Nfkb3, Rela
Sequence domains:
Structure domains:
Nuclear factor NF-kappa-B p50 subunit Chain: C
Molecule details ›
Chain: C
Length: 119 amino acids
Theoretical weight: 13.93 KDa
Source organism: Mus musculus
Expression system: Escherichia coli
UniProt:
  • Canonical: P25799 (Residues: 245-363; Coverage: 12%)
Gene name: Nfkb1
Sequence domains: Rel homology dimerisation domain
Structure domains: Immunoglobulins
NF-kappa-B inhibitor alpha Chain: D
Molecule details ›
Chain: D
Length: 236 amino acids
Theoretical weight: 26.1 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P25963 (Residues: 67-302; Coverage: 74%)
Gene names: IKBA, MAD3, NFKBI, NFKBIA
Sequence domains: Ankyrin repeat
Structure domains: Ankyrin repeat-containing domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 124.5Å b: 49.3Å c: 120.6Å
α: 90° β: 108.7° γ: 90°
R-values:
R R work R free
0.223 0.223 0.277
Expression systems:
  • Escherichia coli
  • Escherichia coli BL21(DE3)

Quick links

Citations

83 review citations

Phosphorylation meets ubiquitination: the control of NF-[kappa]B activity.
Karin et al. (2000)
82 more

82 mentions without citation

A primer on ankyrin repeat function in TRP channels and beyond.
Gaudet R. (2008)
81 more