› 1ikp

X-ray diffraction
1.45Å resolution

Pseudomonas Aeruginosa Exotoxin A, P201Q, W281A mutant

Released:
DOI: 10.2210/pdb1ikp/pdb
PDB entry 1ikp coloured by chain, front view.
PDB entry 1ikp coloured by chain, side view.
PDB entry 1ikp coloured by chain, top view.
Source organism: Pseudomonas aeruginosa
Primary publication:
Refined crystallographic structure of Pseudomonas aeruginosa exotoxin A and its implications for the molecular mechanism of toxicity.
Wedekind JE, Trame CB, Dorywalska M, Koehl P, Raschke TM, McKee M, FitzGerald D, Collier RJ, McKay DB
J Mol Biol 314 823-37 (2001)
PMID: 11734000

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exotoxin A Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 66.79 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11439 (Residues: 26-638; Coverage: 100%)
Gene names: PA1148, eta
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21
Unit cell:
a: 59.91Å b: 99.07Å c: 58.63Å
α: 90° β: 97.34° γ: 90°
R-values:
R R work R free
0.205 0.205 0.228
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

21 review citations

Furin at the cutting edge: from protein traffic to embryogenesis and disease.
Thomas G. (2002)
20 more

2 mentions without citation

Reciprocal carbonyl-carbonyl interactions in small molecules and proteins.
Rahim et al. (2017)
1 more