› 1ikq

X-ray diffraction
1.62Å resolution

Function and Biology Details

Reaction catalysed: 
NAD(+) + diphthamide-[translation elongation factor 2] = nicotinamide + N-(ADP-D-ribosyl)diphthamide-[translation elongation factor 2]
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-145826 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Exotoxin A Chain: A
Molecule details ›
Chain: A
Length: 613 amino acids
Theoretical weight: 66.87 KDa
Source organism: Pseudomonas aeruginosa
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P11439 (Residues: 26-638; Coverage: 100%)
Gene names: PA1148, eta
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CL 2 x CL
Ligand NA 2 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 59.52Å b: 98.12Å c: 58.26Å
α: 90° β: 97.3° γ: 90°
R-values:
R R work R free
0.21 0.209 0.235
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

19 review citations

Furin at the cutting edge: from protein traffic to embryogenesis and disease.
Thomas G. (2002)
18 more

35 mentions without citation

A guide to taming a toxin--recombinant immunotoxins constructed from Pseudomonas exotoxin A for the treatment of cancer.
Weldon et al. (2011)
34 more