› 1im5

X-ray diffraction
1.65Å resolution

Crystal Structure of Pyrazinamidase of Pyrococcus horikoshii in Complex with Zinc

Released:
DOI: 10.2210/pdb1im5/pdb
PDB entry 1im5 coloured by chain, front view.
PDB entry 1im5 coloured by chain, side view.
PDB entry 1im5 coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Crystal structure and mechanism of catalysis of a pyrazinamidase from Pyrococcus horikoshii.
Du X, Wang W, Kim R, Yakota H, Nguyen H, Kim SH
Biochemistry 40 14166-72 (2001)
PMID: 11714269

Function and Biology Details

Reaction catalysed: 
Nicotinamide + H(2)O = nicotinate + NH(3)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129760 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
nicotinamidase Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 20.22 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O58727 (Residues: 1-180; Coverage: 100%)
Gene name: PH0999
Sequence domains: Isochorismatase family
Structure domains: Isochorismatase-like

Ligands and Environments

1 bound ligand:
Ligand ZN 1 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 5.0.2
Spacegroup: P21
Unit cell:
a: 32.978Å b: 43.266Å c: 55.553Å
α: 90° β: 101.34° γ: 90°
R-values:
R R work R free
0.153 0.153 0.232
Expression system: Escherichia coli

Quick links

Citations

5 review citations

Protein acetylation in metabolism - metabolites and cofactors.
Menzies et al. (2016)
4 more

16 mentions without citation

Crystal structure of the pyrazinamidase of Mycobacterium tuberculosis: insights into natural and acquired resistance to pyrazinamide.
Petrella et al. (2011)
15 more