› 1iri

X-ray diffraction
2.4Å resolution

Crystal structure of human autocrine motility factor complexed with an inhibitor

Released:
DOI: 10.2210/pdb1iri/pdb
PDB entry 1iri coloured by chain, front view.
PDB entry 1iri coloured by chain, side view.
PDB entry 1iri coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Inhibition mechanism of cytokine activity of human autocrine motility factor examined by crystal structure analyses and site-directed mutagenesis studies.
Tanaka N, Haga A, Uemura H, Akiyama H, Funasaka T, Nagase H, Raz A, Nakamura KT
J Mol Biol 318 985-97 (2002)
PMID: 12054796

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-139198 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 558 amino acids
Theoretical weight: 63.23 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06744 (Residues: 1-558; Coverage: 100%)
Gene name: GPI
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand E4P 4 x E4P
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 80.651Å b: 107.771Å c: 270.345Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.193 0.241
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Drug development against metastasis-related genes and their pathways: a rationale for cancer therapy.
Iiizumi et al. (2008)
2 more

6 mentions without citation

Protein subunit interfaces: heterodimers versus homodimers.
Zhanhua et al. (2005)
5 more