› 1itw

X-ray diffraction
1.95Å resolution

Crystal structure of the monomeric isocitrate dehydrogenase in complex with isocitrate and Mn

Released:
DOI: 10.2210/pdb1itw/pdb
PDB entry 1itw coloured by chain, front view.
PDB entry 1itw coloured by chain, side view.
PDB entry 1itw coloured by chain, top view.
Source organism: Azotobacter vinelandii
Primary publication:
Structure of the monomeric isocitrate dehydrogenase: evidence of a protein monomerization by a domain duplication.
Yasutake Y, Watanabe S, Yao M, Takada Y, Fukunaga N, Tanaka I
Structure 10 1637-48 (2002)
PMID: 12467571

Function and Biology Details

Reaction catalysed: 
(1a) isocitrate + NADP(+) = 2-oxalosuccinate + NADPH
Biochemical function:
Biological process:
Cellular component:
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-147611 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Isocitrate dehydrogenase [NADP] Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 741 amino acids
Theoretical weight: 80.51 KDa
Source organism: Azotobacter vinelandii
UniProt:
  • Canonical: P16100 (Residues: 1-741; Coverage: 100%)
Gene name: icd
Sequence domains: Monomeric isocitrate dehydrogenase

Ligands and Environments

2 bound ligands:
Ligand MN 5 x MN
Ligand ICT 4 x ICT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 110.405Å b: 119.023Å c: 128.217Å
α: 90° β: 99.006° γ: 90°
R-values:
R R work R free
0.193 0.193 0.228

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Citations

1 review citation

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Tian et al. (2022)
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9 mentions without citation

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