› 1j1d

X-ray diffraction
2.61Å resolution

Crystal structure of the 46kDa domain of human cardiac troponin in the Ca2+ saturated form

Released:
DOI: 10.2210/pdb1j1d/pdb
PDB entry 1j1d coloured by chain, front view.
PDB entry 1j1d coloured by chain, side view.
PDB entry 1j1d coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure of the core domain of human cardiac troponin in the Ca(2+)-saturated form.
Takeda S, Yamashita A, Maeda K, Maéda Y
Nature 424 35-41 (2003)
PMID: 12840750

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148573 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Troponin C, slow skeletal and cardiac muscles Chains: A, D
Molecule details ›
Chains: A, D
Length: 161 amino acids
Theoretical weight: 18.4 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P63316 (Residues: 1-161; Coverage: 100%)
Gene names: TNNC, TNNC1
Sequence domains:
Structure domains: EF-hand
Troponin T, cardiac muscle Chains: B, E
Molecule details ›
Chains: B, E
Length: 106 amino acids
Theoretical weight: 12.84 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P45379 (Residues: 193-298; Coverage: 36%)
Gene name: TNNT2
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Troponin I, cardiac muscle Chains: C, F
Molecule details ›
Chains: C, F
Length: 133 amino acids
Theoretical weight: 15.19 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P19429 (Residues: 31-163; Coverage: 63%)
Gene names: TNNC1, TNNI3
Sequence domains: Troponin
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand CA 6 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL41XU
Spacegroup: P21
Unit cell:
a: 42.346Å b: 167.86Å c: 69.707Å
α: 90° β: 101.35° γ: 90°
R-values:
R R work R free
0.264 0.264 0.298
Expression system: Escherichia coli

Quick links

Citations

100 review citations

Troponin: the biomarker of choice for the detection of cardiac injury.
Babuin et al. (2005)
99 more

38 mentions without citation

Troponin structure and function: a view of recent progress.
Marston et al. (2020)
37 more