› 1j3w

X-ray diffraction
1.5Å resolution

Structure of Gliding protein-mglB from Thermus Thermophilus HB8

Released:
DOI: 10.2210/pdb1j3w/pdb
PDB entry 1j3w coloured by chain, front view.
PDB entry 1j3w coloured by chain, side view.
PDB entry 1j3w coloured by chain, top view.
Source organism: Thermus thermophilus HB8
Entry authors: Lokanath NK, Kunishima N, Miyano M, RIKEN Structural Genomics/Proteomics Initiative (RSGI)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-194966 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Roadblock/LAMTOR2 domain-containing protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 163 amino acids
Theoretical weight: 17.81 KDa
Source organism: Thermus thermophilus HB8
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9X9L0 (Residues: 1-163; Coverage: 100%)
Gene names: TthHB5018_12140, mglB
Sequence domains: Roadblock/LC7 domain
Structure domains: Dynein light chain 2a, cytoplasmic

Ligands and Environments

3 bound ligands:
Ligand SO4 2 x SO4
Ligand MES 2 x MES
Ligand MG 1 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL26B1
Spacegroup: C2221
Unit cell:
a: 99.982Å b: 117.186Å c: 111.467Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.208 0.222
Expression system: Escherichia coli BL21(DE3)

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