› 1j7l

X-ray diffraction
2.2Å resolution

Crystal Structure of 3',5"-Aminoglycoside Phosphotransferase Type IIIa ADP Complex

Released:
DOI: 10.2210/pdb1j7l/pdb
PDB entry 1j7l coloured by chain, front view.
PDB entry 1j7l coloured by chain, side view.
PDB entry 1j7l coloured by chain, top view.
Source organism: Enterococcus faecalis
Primary publication:
Structural analyses of nucleotide binding to an aminoglycoside phosphotransferase.
Burk DL, Hon WC, Leung AK, Berghuis AM
Biochemistry 40 8756-64 (2001)
PMID: 11467935

Function and Biology Details

Reaction catalysed: 
ATP + kanamycin = ADP + kanamycin 3'-phosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aminoglycoside 3'-phosphotransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 264 amino acids
Theoretical weight: 31.01 KDa
Source organism: Enterococcus faecalis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A3Y5 (Residues: 1-264; Coverage: 100%)
Gene name: aphA
Sequence domains: Phosphotransferase enzyme family
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 4 x MG
Ligand ADP 2 x ADP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P212121
Unit cell:
a: 49.71Å b: 91.245Å c: 131.301Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.22 0.22 0.279
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Molecular understanding of aminoglycoside action and resistance.
Jana et al. (2006)
8 more

6 mentions without citation

Aminoglycoside modifying enzymes.
Ramirez et al. (2010)
5 more