› 1j84

X-ray diffraction
2.02Å resolution

STRUCTURE OF FAM17 CARBOHYDRATE BINDING MODULE FROM CLOSTRIDIUM CELLULOVORANS WITH BOUND CELLOTETRAOSE

Released:
DOI: 10.2210/pdb1j84/pdb
PDB entry 1j84 coloured by chain, front view.
PDB entry 1j84 coloured by chain, side view.
PDB entry 1j84 coloured by chain, top view.
Source organism: Clostridium cellulovorans
Primary publication:
Recognition of cello-oligosaccharides by a family 17 carbohydrate-binding module: an X-ray crystallographic, thermodynamic and mutagenic study.
Notenboom V, Boraston AB, Chiu P, Freelove AC, Kilburn DG, Rose DR
J Mol Biol 314 797-806 (2001)
PMID: 11733998

Function and Biology Details

Reaction catalysed: 
Endohydrolysis of (1->4)-beta-D-glucosidic linkages in cellulose, lichenin and cereal beta-D-glucans
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161122 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
cellulase Chain: A
Molecule details ›
Chain: A
Length: 180 amino acids
Theoretical weight: 19.32 KDa
Source organism: Clostridium cellulovorans
Expression system: Escherichia coli
UniProt:
  • Canonical: P94622 (Residues: 378-557; Coverage: 34%)
Gene name: engF
Sequence domains: Carbohydrate binding domain (family 17/28)
Structure domains: Galactose-binding domain-like

Ligands and Environments

Carbohydrate polymer : NEW Components: BGC
Carbohydrate polymer
1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P6122
Unit cell:
a: 69.095Å b: 69.095Å c: 143.59Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.225 0.225 0.264
Expression system: Escherichia coli

Quick links

Citations

3 review citations

Advances in understanding the molecular basis of plant cell wall polysaccharide recognition by carbohydrate-binding modules.
Gilbert et al. (2013)
2 more

3 mentions without citation

Carbohydrate-binding modules: fine-tuning polysaccharide recognition.
Boraston et al. (2004)
2 more