Function and Biology Details
Reaction catalysed:
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu)
Biochemical function:
Biological process:
Cellular component:
Structure analysis Details
Assembly composition:
homo dimer (preferred)
Assembly name:
tRNA (cytidine(34)-2'-O)-methyltransferase (preferred)
PDBe Complex ID:
PDB-CPX-155219 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (cytidine(34)-2'-O)-methyltransferase
Molecule details ›
Chain: A
Length: 160 amino acids
Theoretical weight: 18.43 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
UniProt:
Sequence domains: SpoU rRNA Methylase family
Structure domains: Alpha/beta knot
Length: 160 amino acids
Theoretical weight: 18.43 KDa
Source organism: Haemophilus influenzae Rd KW20
Expression system: Escherichia coli BL21(DE3)
UniProt:
- Canonical:
P44868 (Residues: 1-160; Coverage: 100%)
Sequence domains: SpoU rRNA Methylase family
Structure domains: Alpha/beta knot
Ligands and Environments
No bound ligands
No modified residues
Experiments and Validation Details
X-ray source:
APS BEAMLINE 17-ID
Spacegroup:
P41212
Expression system: Escherichia coli BL21(DE3)
