› 1jc0

X-ray diffraction
2Å resolution

CRYSTAL STRUCTURE ANALYSIS OF A REDOX-SENSITIVE GREEN FLUORESCENT PROTEIN VARIANT IN A REDUCED FORM

Released:
DOI: 10.2210/pdb1jc0/pdb
PDB entry 1jc0 coloured by chain, front view.
PDB entry 1jc0 coloured by chain, side view.
PDB entry 1jc0 coloured by chain, top view.
Source organism: Aequorea victoria
Primary publication:
Investigating mitochondrial redox potential with redox-sensitive green fluorescent protein indicators.
Hanson GT, Aggeler R, Oglesbee D, Cannon M, Capaldi RA, Tsien RY, Remington SJ
J Biol Chem 279 13044-53 (2004)
PMID: 14722062

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-154537 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 236 amino acids
Theoretical weight: 26.89 KDa
Source organism: Aequorea victoria
Expression system: Escherichia coli
UniProt:
  • Canonical: P42212 (Residues: 1-238; Coverage: 100%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

No bound ligands
1 modified residue:
Ligand CRO 3 x CRO

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21212
Unit cell:
a: 185.63Å b: 67.862Å c: 56.376Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.223 0.223 not available
Expression system: Escherichia coli

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Citations

166 review citations

Oxidative Stress.
Sies et al. (2017)
165 more

3 mentions without citation

Intracellular CXCR4⁺ cell targeting with T22-empowered protein-only nanoparticles.
Unzueta et al. (2012)
2 more