› 1jca

X-ray diffraction
2.5Å resolution

Non-standard Design of Unstable Insulin Analogues with Enhanced Activity

Released:
DOI: 10.2210/pdb1jca/pdb
PDB entry 1jca coloured by chain, front view.
PDB entry 1jca coloured by chain, side view.
PDB entry 1jca coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Non-standard insulin design: structure-activity relationships at the periphery of the insulin receptor.
Weiss MA, Wan Z, Zhao M, Chu YC, Nakagawa SH, Burke GT, Jia W, Hellmich R, Katsoyannis PG
J Mol Biol 315 103-11 (2002)
PMID: 11779231

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
hetero hexamer (preferred)
hetero dodecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-134224 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Insulin A chain Chains: A, C
Molecule details ›
Chains: A, C
Length: 21 amino acids
Theoretical weight: 2.41 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 90-110; Coverage: 24%)
Gene name: INS
Insulin B chain Chains: B, D
Molecule details ›
Chains: B, D
Length: 30 amino acids
Theoretical weight: 3.43 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: P01308 (Residues: 25-54; Coverage: 35%)
Gene name: INS
Sequence domains: Insulin/IGF/Relaxin family

Ligands and Environments

1 bound ligand:
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 80.652Å b: 80.652Å c: 37.936Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.204 0.204 0.241
Expression system: Not provided

Quick links

Citations

2 review citations

A Review of the Biosynthesis and Structural Implications of Insulin Gene Mutations Linked to Human Disease.
Ataie-Ashtiani et al. (2023)
1 more