1je8

X-ray diffraction
2.12Å resolution

Two-Component response regulator NarL/DNA Complex: DNA Bending Found in a High Affinity Site

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-113998 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Nitrate/nitrite response regulator protein NarL Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 82 amino acids
Theoretical weight: 9.83 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AF28 (Residues: 147-216; Coverage: 32%)
Gene names: JW1212, b1221, frdR, narL
Sequence domains: Bacterial regulatory proteins, luxR family
Structure domains: Winged helix-like DNA-binding domain superfamily/Winged helix DNA-binding domain
5'-D(*CP*GP*TP*AP*CP*CP*CP*AP*TP*TP*AP*AP*TP*GP*GP*GP*TP*AP*CP*G)-3' Chains: C, D, G, H
Molecule details ›
Chains: C, D, G, H
Length: 20 nucleotides
Theoretical weight: 6.13 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

1 bound ligand:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X8C
Spacegroup: P21
Unit cell:
a: 76.257Å b: 52.741Å c: 83.863Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.228 0.228 0.273
Expression systems:
  • Escherichia coli
  • Not provided