› 1jf5

X-ray diffraction
3.2Å resolution

Function and Biology Details

Reaction catalysed: 
Hydrolysis of pullulan to panose (6-alpha-D-glucosylmaltose)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-170689 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Neopullulanase 2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 585 amino acids
Theoretical weight: 67.48 KDa
Source organism: Thermoactinomyces vulgaris
Expression system: Escherichia coli
UniProt:
  • Canonical: Q08751 (Residues: 1-585; Coverage: 100%)
Gene name: tvaII
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PHOTON FACTORY BEAMLINE BL-6A
Spacegroup: P212121
Unit cell:
a: 112.6Å b: 118.05Å c: 113.13Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.214 0.214 0.273
Expression system: Escherichia coli

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Citations

2 review citations

Structure and function of α-glucan debranching enzymes.
Møller et al. (2016)
1 more