› 1jfg

X-ray diffraction
2.5Å resolution

TRICHODIENE SYNTHASE FROM FUSARIUM SPOROTRICHIOIDES COMPLEXED WITH DIPHOSPHATE

Released:
DOI: 10.2210/pdb1jfg/pdb
PDB entry 1jfg coloured by chain, front view.
PDB entry 1jfg coloured by chain, side view.
PDB entry 1jfg coloured by chain, top view.
Source organism: Fusarium sporotrichioides
Primary publication:
Structure of trichodiene synthase from Fusarium sporotrichioides provides mechanistic inferences on the terpene cyclization cascade.
Rynkiewicz MJ, Cane DE, Christianson DW
Proc Natl Acad Sci U S A 98 13543-8 (2001)
PMID: 11698643

Function and Biology Details

Reaction catalysed: 
(2E,6E)-farnesyl diphosphate = trichodiene + diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146631 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Trichodiene synthase Chains: A, B
Molecule details ›
Chains: A, B
Length: 374 amino acids
Theoretical weight: 44.05 KDa
Source organism: Fusarium sporotrichioides
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P13513 (Residues: 1-374; Coverage: 100%)
Gene names: TOX 5, TRI5
Sequence domains: Trichodiene synthase (TRI5)
Structure domains: Farnesyl Diphosphate Synthase

Ligands and Environments

3 bound ligands:
Ligand GOL 3 x GOL
Ligand MG 3 x MG
Ligand POP 1 x POP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P3121
Unit cell:
a: 122.574Å b: 122.574Å c: 150.869Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.217 0.217 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

27 review citations

Fungal secondary metabolism - from biochemistry to genomics.
Keller et al. (2005)
26 more

8 mentions without citation

Terpenoid synthase structures: a so far incomplete view of complex catalysis.
Gao et al. (2012)
7 more