› 1jft

X-ray diffraction
2.5Å resolution

PURINE REPRESSOR MUTANT-HYPOXANTHINE-PURF OPERATOR COMPLEX

Released:
DOI: 10.2210/pdb1jft/pdb
PDB entry 1jft coloured by chain, front view.
PDB entry 1jft coloured by chain, side view.
PDB entry 1jft coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor.
Huffman JL, Lu F, Zalkin H, Brennan RG
Biochemistry 41 511-20 (2002)
PMID: 11781089

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114003 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
HTH-type transcriptional repressor PurR Chain: A
Molecule details ›
Chain: A
Length: 340 amino acids
Theoretical weight: 37.98 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACP7 (Residues: 2-341; Coverage: 100%)
Gene names: JW1650, b1658, purR
Sequence domains:
Structure domains:
5'-D(*TP*AP*CP*GP*CP*AP*AP*AP*CP*GP*TP*TP*TP*GP*CP*GP*T)-3' Chain: B
Molecule details ›
Chain: B
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: Escherichia coli
Expression system: Not provided

Ligands and Environments

2 bound ligands:
Ligand PO4 1 x PO4
Ligand HPA 1 x HPA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 175.918Å b: 95.25Å c: 81.349Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.192 not available not available
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

1 review citation

Allostery in the LacI/GalR family: variations on a theme.
Swint-Kruse et al. (2009)
10 other articles

5 mentions without citation

DNA conformations and their sequence preferences.
Svozil et al. (2008)
4 more