› 1jfv

X-ray diffraction
2Å resolution

X-Ray Structure of oxidised C10S, C15A arsenate reductase from pI258

Released:
DOI: 10.2210/pdb1jfv/pdb
PDB entry 1jfv coloured by chain, front view.
PDB entry 1jfv coloured by chain, side view.
PDB entry 1jfv coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
Arsenate reductase from S. aureus plasmid pI258 is a phosphatase drafted for redox duty.
Zegers I, Martins JC, Willem R, Wyns L, Messens J
Nat Struct Biol 8 843-7 (2001)
PMID: 11573087

Function and Biology Details

Reaction catalysed: 
Arsenate + thioredoxin = arsenite + thioredoxin disulfide + H(2)O
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140882 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Arsenate reductase Chain: A
Molecule details ›
Chain: A
Length: 131 amino acids
Theoretical weight: 14.88 KDa
Source organism: Staphylococcus aureus
Expression system: Escherichia coli
UniProt:
  • Canonical: P0A006 (Residues: 1-131; Coverage: 100%)
Gene name: arsC
Sequence domains: Low molecular weight phosphotyrosine protein phosphatase
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand LCP 1 x LCP
Ligand K 1 x K
1 modified residue:
Ligand MSE 2 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE BW7A
Spacegroup: P212121
Unit cell:
a: 33.8Å b: 35.63Å c: 109.53Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.236 0.236 0.286
Expression system: Escherichia coli

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Citations

11 review citations

Bacterial silver resistance: molecular biology and uses and misuses of silver compounds.
Silver S. (2003)
10 more

2 mentions without citation

Alternate states of proteins revealed by detailed energy landscape mapping.
Tyka et al. (2011)
1 more