› 1jgi

X-ray diffraction
2Å resolution

Crystal Structure of the Active Site Mutant Glu328Gln of Amylosucrase from Neisseria polysaccharea in Complex with the Natural Substrate Sucrose

Released:
DOI: 10.2210/pdb1jgi/pdb
PDB entry 1jgi coloured by chain, front view.
PDB entry 1jgi coloured by chain, side view.
PDB entry 1jgi coloured by chain, top view.
Source organism: Neisseria polysaccharea
Primary publication:
Crystal structures of amylosucrase from Neisseria polysaccharea in complex with D-glucose and the active site mutant Glu328Gln in complex with the natural substrate sucrose.
Mirza O, Skov LK, Remaud-Simeon M, Potocki de Montalk G, Albenne C, Monsan P, Gajhede M
Biochemistry 40 9032-9 (2001)
PMID: 11467966

Function and Biology Details

Reaction catalysed: 
Sucrose + ((1->4)-alpha-D-glucosyl)(n) = D-fructose + ((1->4)-alpha-D-glucosyl)(n+1)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195625 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Amylosucrase Chain: A
Molecule details ›
Chain: A
Length: 628 amino acids
Theoretical weight: 71.59 KDa
Source organism: Neisseria polysaccharea
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9ZEU2 (Residues: 13-636; Coverage: 98%)
Gene name: ams
Sequence domains: Alpha amylase, catalytic domain
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: GLC, FRU
Carbohydrate polymer
No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MAX II BEAMLINE I711
Spacegroup: P21212
Unit cell:
a: 95.588Å b: 116.739Å c: 60.833Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.226
Expression system: Escherichia coli

Quick links

Citations

6 review citations

Structure-function relationships of glucansucrase and fructansucrase enzymes from lactic acid bacteria.
van Hijum et al. (2006)
5 more

9 mentions without citation

Characterization of the highly efficient sucrose isomerase from Pantoea dispersa UQ68J and cloning of the sucrose isomerase gene.
Wu et al. (2005)
8 more