› 1jhd

X-ray diffraction
1.7Å resolution

Crystal Structure of Bacterial ATP Sulfurylase from the Riftia pachyptila Symbiont

Released:
DOI: 10.2210/pdb1jhd/pdb
PDB entry 1jhd coloured by chain, front view.
PDB entry 1jhd coloured by chain, side view.
PDB entry 1jhd coloured by chain, top view.
Source organism: sulfur-oxidizing endosymbiont of Riftia pachyptila
Primary publication:
Crystal structure of ATP sulfurylase from the bacterial symbiont of the hydrothermal vent tubeworm Riftia pachyptila.
Beynon JD, MacRae IJ, Huston SL, Nelson DC, Segel IH, Fisher AJ
Biochemistry 40 14509-17 (2001)
PMID: 11724564

Function and Biology Details

Reaction catalysed: 
ATP + sulfate = diphosphate + adenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-176211 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Sulfate adenylyltransferase Chain: A
Molecule details ›
Chain: A
Length: 396 amino acids
Theoretical weight: 43.94 KDa
Source organism: sulfur-oxidizing endosymbiont of Riftia pachyptila
Expression system: Escherichia coli
UniProt:
  • Canonical: Q54506 (Residues: 1-54, 55-77, 78-297, 298-317, 318-386; Coverage: 87%)
Gene names: sat, sopT
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 3 x SO4
Ligand BR 8 x BR
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P21212
Unit cell:
a: 59.561Å b: 75.546Å c: 95.853Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.167 0.167 0.205
Expression system: Escherichia coli

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Citations

6 review citations

Chemosynthetic endosymbioses: adaptations to oxic-anoxic interfaces.
Stewart et al. (2005)
5 more

4 mentions without citation

Structural, biochemical and genetic characterization of dissimilatory ATP sulfurylase from Allochromatium vinosum.
Parey et al. (2013)
3 more