› 1jhz

X-ray diffraction
2.4Å resolution

Purine Repressor Mutant Corepressor Binding Domain Structure

Released:
DOI: 10.2210/pdb1jhz/pdb
PDB entry 1jhz coloured by chain, front view.
PDB entry 1jhz coloured by chain, side view.
PDB entry 1jhz coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
Role of residue 147 in the gene regulatory function of the Escherichia coli purine repressor.
Huffman JL, Lu F, Zalkin H, Brennan RG
Biochemistry 41 511-20 (2002)
PMID: 11781089

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142291 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
HTH-type transcriptional repressor PurR Chains: A, B
Molecule details ›
Chains: A, B
Length: 289 amino acids
Theoretical weight: 32.45 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACP7 (Residues: 53-341; Coverage: 85%)
Gene names: JW1650, b1658, purR
Sequence domains: Periplasmic binding protein-like domain
Structure domains: Rossmann fold

Ligands and Environments

1 bound ligand:
Ligand MG 3 x MG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL7-1
Spacegroup: P21
Unit cell:
a: 38.02Å b: 126.13Å c: 61.84Å
α: 90° β: 99.71° γ: 90°
R-values:
R R work R free
0.178 not available not available
Expression system: Escherichia coli

Quick links

Citations

1 review citation

Allostery in the LacI/GalR family: variations on a theme.
Swint-Kruse et al. (2009)
9 other articles

1 mention without citation

Automated selection of positions determining functional specificity of proteins by comparative analysis of orthologous groups in protein families.
Kalinina et al. (2004)