› 1jid

X-ray diffraction
1.8Å resolution

Human SRP19 in complex with helix 6 of Human SRP RNA

Released:
DOI: 10.2210/pdb1jid/pdb
PDB entry 1jid coloured by chain, front view.
PDB entry 1jid coloured by chain, side view.
PDB entry 1jid coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of an early protein-RNA assembly complex of the signal recognition particle.
Wild K, Sinning I, Cusack S
Science 294 598-601 (2001)
PMID: 11641499

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140559 (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct RNA molecule
Macromolecules (2 distinct):
Signal recognition particle 19 kDa protein Chain: A
Molecule details ›
Chain: A
Length: 128 amino acids
Theoretical weight: 14.79 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P09132 (Residues: 1-120; Coverage: 83%)
Gene name: SRP19
Sequence domains: SRP19 protein
Structure domains: Signal recognition particle, SRP19-like subunit
HELIX 6 OF HUMAN SRP RNA Chain: B
Molecule details ›
Chain: B
Length: 29 nucleotides
Theoretical weight: 9.63 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand MG 2 x MG
2 modified residues:
Ligand 5BU 2 x 5BU
Ligand A23 1 x A23

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: C2221
Unit cell:
a: 55.813Å b: 109.782Å c: 89.461Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.186 0.186 0.222
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

17 review citations

Sorting it out: regulation of exosome loading.
Villarroya-Beltri et al. (2014)
16 more

21 mentions without citation

Computational approaches to 3D modeling of RNA.
Laing et al. (2010)
20 more