› 1jj6

X-ray diffraction
2.3Å resolution

Testing the Water-Mediated Hin Recombinase DNA Recognition by Systematic Mutations.

Released:
DOI: 10.2210/pdb1jj6/pdb
PDB entry 1jj6 coloured by chain, front view.
PDB entry 1jj6 coloured by chain, side view.
PDB entry 1jj6 coloured by chain, top view.
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Primary publication:
Testing water-mediated DNA recognition by the Hin recombinase.
Chiu TK, Sohn C, Dickerson RE, Johnson RC
EMBO J 21 801-14 (2002)
PMID: 11847127

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-114009 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-invertase hin Chain: C
Molecule details ›
Chain: C
Length: 52 amino acids
Theoretical weight: 6.05 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
UniProt:
  • Canonical: P03013 (Residues: 139-190; Coverage: 27%)
Gene names: STM2772, hin
Sequence domains: Helix-turn-helix domain of resolvase
Structure domains: Homeodomain-like
5'-D(*TP*GP*TP*(5IT)P*TP*TP*TP*GP*AP*TP*AP*AP*GP*A)-3' Chain: A
Molecule details ›
Chain: A
Length: 14 nucleotides
Theoretical weight: 4.44 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided
5'-D(*AP*TP*CP*TP*TP*AP*TP*CP*AP*AP*AP*AP*AP*C)-3' Chain: B
Molecule details ›
Chain: B
Length: 14 nucleotides
Theoretical weight: 4.23 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand TRS 1 x TRS
1 modified residue:
Ligand 5IU 1 x 5IU

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: C2221
Unit cell:
a: 86.796Å b: 82.12Å c: 45.234Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.238 0.281
Expression system: Not provided

Quick links

Citations

1 review citation

The role of water in protein-DNA recognition.
Jayaram et al. (2004)
17 other articles

3 mentions without citation

Probing the role of interfacial waters in protein-DNA recognition using a hybrid implicit/explicit solvation model.
Li et al. (2013)
2 more