› 1jk7

X-ray diffraction
1.9Å resolution

CRYSTAL STRUCTURE OF THE TUMOR-PROMOTER OKADAIC ACID BOUND TO PROTEIN PHOSPHATASE-1

Released:
DOI: 10.2210/pdb1jk7/pdb
PDB entry 1jk7 coloured by chain, front view.
PDB entry 1jk7 coloured by chain, side view.
PDB entry 1jk7 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Crystal structure of the tumor-promoter okadaic acid bound to protein phosphatase-1.
Maynes JT, Bateman KS, Cherney MM, Das AK, Luu HA, Holmes CF, James MN
J Biol Chem 276 44078-82 (2001)
PMID: 11535607

Function and Biology Details

Reaction catalysed: 
[a protein]-serine/threonine phosphate + H(2)O = [a protein]-serine/threonine + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153234 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit Chain: A
Molecule details ›
Chain: A
Length: 323 amino acids
Theoretical weight: 37.03 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P36873 (Residues: 1-323; Coverage: 100%)
Gene name: PPP1CC
Sequence domains:
Structure domains: Purple Acid Phosphatase; chain A, domain 2

Ligands and Environments

4 bound ligands:
Ligand MN 2 x MN
Ligand SO4 1 x SO4
Ligand OKA 1 x OKA
Ligand BME 3 x BME
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RUH3R
Spacegroup: P42212
Unit cell:
a: 99.18Å b: 99.18Å c: 62.17Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.199 0.225
Expression system: Escherichia coli

Quick links

Citations

17 review citations

Functional diversity of protein phosphatase-1, a cellular economizer and reset button.
Ceulemans et al. (2004)
16 more

14 mentions without citation

Structural basis for protein phosphatase 1 regulation and specificity.
Peti et al. (2013)
13 more