› 1jms

X-ray diffraction
2.36Å resolution

Crystal Structure of the Catalytic Core of Murine Terminal Deoxynucleotidyl Transferase

Released:
DOI: 10.2210/pdb1jms/pdb
PDB entry 1jms coloured by chain, front view.
PDB entry 1jms coloured by chain, side view.
PDB entry 1jms coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Crystal structures of a template-independent DNA polymerase: murine terminal deoxynucleotidyltransferase.
Delarue M, Boulé JB, Lescar J, Expert-Bezançon N, Jourdan N, Sukumar N, Rougeon F, Papanicolaou C
EMBO J 21 427-39 (2002)
PMID: 11823435

Function and Biology Details

Reaction catalysed: 
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-140763 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
DNA nucleotidylexotransferase Chain: A
Molecule details ›
Chain: A
Length: 381 amino acids
Theoretical weight: 43.6 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P09838 (Residues: 130-510; Coverage: 75%)
Gene names: Dntt, Tdt
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand MG 1 x MG
Ligand NA 1 x NA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-3
Spacegroup: P212121
Unit cell:
a: 47.1Å b: 85.2Å c: 111.7Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.218 0.214 0.259
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

15 review citations

Repair of double-strand breaks by end joining.
Chiruvella et al. (2013)
14 more

15 mentions without citation

The X family portrait: structural insights into biological functions of X family polymerases.
Moon et al. (2007)
14 more